May 20, 2020 I Jola Glotzer
A “quick and easy” COVID-19 test
This is a goal of NU scientists, including two past CBC awardees Michael Jewett and Joshua Leonard, who are co-PIs on a new NSF RAPID grant aiming to develop a one-stop infectious diseases testing device
Congratulations to a NU team of synthetic biology researchers for securing the National Science Foundation’s (NSF) Rapid Response Research (RAPID) grant, which provides support to the projects that address COVID-19-related research questions. The NU team includes two CBC awardees, Michael Jewett and Joshua Leonard, NU, who are co-PIs on the project. Their idea is to develop an easy-to-use cell-free biosensor-type test capable of detecting infectious diseases, including COVID-19. Ideally, the test should be as simple and accessible to the general public as, e.g., a pregnancy test (see below).
Jewett and Leonard have multiple ties to the CBC. Both received CBC Catalyst Awards in the past and were invited to speak at the Annual CBC Symposia. In addition, since 2016, Jewett has been serving on the CBC Catalyst Review Board (see below). Thank you!
The CBC is proud to have supported Jewett’s and Leonard’s earlier research and wishes them all the best in their current quest to develop this new and much needed testing device.
May 20, 2020 | By Amanda Morris | Northwestern Now
One-step diagnostic tool receives NSF RAPID grant
Rapid, easy, point-of-need test could enable global tracking of infectious diseases
Northwestern University synthetic biologists have received funding to develop an easy-to-use, quick-screen technology that can test for infectious diseases, including COVID-19, in the human body or within the environment.
Similar to a pregnancy test, the tool uses one sample to provide an easy-to-read negative or positive result. By simplifying testing, the researchers could put diagnostics into the hands of people everywhere — without the need for expensive laboratories or expertise. This could provide the large-scale testing required for ending stay-at-home orders, reopening the economy or preparing for a predicted virus resurgence in the fall.
The team is working to develop and optimize the test so that it will be a single step, taking less than an hour to provide a result and less than a dollar to manufacture.
On Friday, the project received a rapid response research (RAPID) grant from the National Science Foundation (NSF), which has called for immediate proposals that have potential to address the spread of novel coronavirus (COVID-19). The grant provides $200,000 over one year.
“The current COVID-19 pandemic highlights the limitations of laboratory-based testing,” said Northwestern’s Julius Lucks, the principal investigator on the project. “Those tests have not scaled with the sudden and dramatic increase of needed volume. They require too much equipment, time, expertise and infrastructure, which have resulted in major logistical challenges and, ultimately, inadequate testing. It’s become clear that we need to dramatically increase the scale of testing to safely restart the economy, to provide critical information if the virus resurges and to provide monitoring to prevent this in the future.”
Lucks devised the project with fellow Northwestern Center for Synthetic Biology (CSB) members Michael Jewett, Joshua Leonard and Niall Mangan. Jewett and Leonard are professors of chemical and biological engineering in Northwestern’s McCormick School of Engineering; Mangan is an assistant professor of engineering sciences and applied mathematics in McCormick. They have partnered with Khalid Alam, a former postdoctoral fellow in Lucks’ laboratory and CEO of Stemloop, Inc., which spun out of the CSB to commercialize rapid, field-deployable synthetic biology diagnostics technologies.
“Over the past few years, we have pioneered the use of cell-free systems as easy-to-use, point-of-need diagnostics,” said Jewett, director of the CSB. “These field-deployable systems can sense a variety of contaminants relevant to public health, including lead, fluoride and atrazine. We are trying to use that know-how to help address gaps in COVID-19 testing.”
The test works by combining gene-editing tool CRISPR, custom genetic circuits and cell-free synthetic biology in order to detect the virus and signal its presence. The team envisions that the final product could test patients’ samples from a nasal swab or saliva sample as well as water and surfaces in the surrounding environment.
“As far as we know, this is the first diagnostic tool to confront environmental monitoring,” Lucks said. “We could use this technology to monitor the presence of the virus on surfaces or even in the water. Recent research suggests that monitoring wastewater might provide clues about viral spread. Given the broad-scale need, we also plan to aim for clinical applications.”
After the test is developed in the laboratory, Stemloop will quickly transition the technology for manufacturing, deployment and distribution. The researchers aim to have the test ready for the novel coronavirus’ predicted resurgence in the fall. The tool, however, also can be rapidly reprogrammed to detect new emerging pathogens for future pandemics.
Lucks, Jewett and Leonard are members of Northwestern’s Chemistry of Life Processes Institute. Jewett and Leonard are members of the Robert H. Lurie Comprehensive Cancer Center of Northwestern University. Alam is an entrepreneur in Argonne National Laboratory’s Chain Reaction Innovations program.
Adapted (with modifications) from Northwestern Now, by Amanda Morris, published on May 20, 2020.
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