May 8, 2019 | Jola Glotzer
Dual cancer therapies
Or, how inhibiting TGF-beta/CD73 pathway reverses an inhibition of anti-tumor T-cell activity. CBC Awardee, Bin Zhang, NU, explains.
Cancer immunotherapy, an approach with great promise in treatment of several types of cancer, has fallen short of demonstrating universal impact, with some patients responding well but many others showing resistance. In some cases the problem seems to be T-cell-specific and can be characterized by T-cells being surprisingly inactive in recognizing and attacking cancer cells. In a recent paper published in Nature Communications, the Zhang group from NU shows that, at least is some cases, T cells can be “woken up” and stimulated to start recognizing and killing cancer cells by inhibiting TGF-beta/CD73 pathway. Both TGF-beta and CD73 are elevated in certain tumors and, once inhibited in animal tumor models, cause a remarkable tumor shrinkage in response to standard immunotherapy applied in parallel. As measuring TGF-beta and CD73 in tumor biopsies is easy, they could become biomarkers allowing to select tumors that could benefit from this double therapeutic intervention. Bin Zhang is senior author on the publication and Lei Qin, a postdoctoral fellow in his lab, is one of the co-authors. Zhang and Qin received a CBC HTS Award in 2014 for the project “Development of Small Molecule CD73 Inhibitors for Cancer Therapy.” CBC funding is acknowledged in the Nature Communications study. Congratulations!
Solving Resistance to Emerging Cancer Immunotherapy
NU Medicine News | by Will Doss, | February 7, 2019
Northwestern scientists have pinpointed how an ectoenzyme called CD73 undermines the effectiveness of an emerging cancer immunotherapy, and demonstrated a method to reduce CD73’s expression and enzymatic activity, according to a study published in Nature Communications.
This could increase agonist therapy’s ability to fight tumors in some patients with cancer, according to Bin Zhang, MD, PhD, professor of Medicine in the Division of Hematology and Oncology and senior author of the study.
“Some cancer patients respond to agonist therapy pretty well, but the majority do not,” said Zhang, who is also a professor of Microbiology-Immunology and a member of the Robert H. Lurie Comprehensive Cancer Center of Northwestern University. “With this therapy, you can improve T-cell function and hopefully achieve the best outcome for patients.”
Agonist therapy relies on activating immune cells that are often suppressed in cancer, killing tumor cells with fewer harmful side effects compared to other treatments such as chemotherapy. Agonist therapy targets co-stimulatory molecules on the surface of T-cells to jump-start immune activity, but pre-clinical and clinical trials have shown limited benefits thus far.
Zhang and his collaborators investigated why some patients respond to a therapy called 4-1BB (CD137) agonist antibodies, while others do not. The scientists took a closer look at CD73, a well-known immunosuppressive ectoenzyme. Analyzing expression levels in multiple mouse tumor models, they discovered that while tumors with low levels of CD73 responded well to therapy, tumors with high levels of CD73 remained resistant, with little anti-tumor T-cell activity.
The investigators traced high levels of CD73 to a tumor growth factor called TGF-beta, determining that this growth factor was sustaining CD73 and therefore interfering with the agonist therapy.
TGF-beta has been on immunologists’ radar for some time, so simple inhibitors are widely available, Zhang said. The scientists proceeded to treat mice with both the agonist therapy and the anti-TGF-beta inhibitor, finding the combination successfully induced tumor regression.
“By blocking TGF-beta, you can shift from the treatment-resistant model to the treatment-sensitive model,” Zhang said.
CD73 is not the only enzyme that breeds resistance to agonist therapy, and this strategy could be used to improve treatment in those cancers as well. Further, this dual therapy regimen could be used in conjunction with other therapies altogether, Zhang said.
“The whole field has tilted towards immunotherapy, but the tumor microenvironment is very complicated,” Zhang said. “It’s not a single mechanism. The best therapy is going to be different from case to case.”
Zhang believes this brand of personalized medicine — typified by Lurie Cancer Center’s OncoSET program — could vastly improve cancer therapy, and the dual-treatment strategy in the current study is a strong illustration of the concept. The drugs are already being used in patients, easing the bench-to-bedside transition, and both TGF-beta and CD73 levels can be detected from a biopsy, making it easy to identify patients who would benefit.
“This treatment is really beautiful in terms of its translational relevance,” Zhang said.
Siqi Chen, PhD, a postdoctoral fellow in Zhang’s laboratory, was the first author of the study, and Lei Qin, postdoctoral fellow and Jeffrey Sosman, MD, professor of Medicine in the Division of Hematology and Oncology, were co-authors.
This study was in part supported by National Institutes of Health grant CA149669, CA208354, and CA222963; Melanoma Research Alliance Pilot Award, the Marsha Rivkin Center for Ovarian Cancer Research Pilot Award, CBC HTS Supplemental Grant, Northwestern University RHLCCC Flow Cytometry Facility, a Cancer Center Support Grant CA060553, the Robert H. Lurie Comprehensive Cancer Center and the National Natural Science Foundation of China grant 81725004.
*Publication attributed to the CBC funding:
Chen S, Fan J, Zhang M, Qin L, Dominguez D, Long A, Wang G, Ma R, Li H, Zhang Y, Fang D, Sosman J, Zhang B. CD73 expression on effector T cells sustained by TGF-β facilitates tumor resistance to anti-4-1BB/CD137 therapy. Nat Commun. 2019 Jan 11;10(1):150. (PubMed)
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